New larval agar-based diet for laboratory rearing of Mediterranean fruit fly <Emphasis Type="Italic">Ceratitis capitata</Emphasis> (Diptera,Tephritidae) |
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Authors: | Martina Pa?ková |
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Institution: | (1) Institute of Zoology SAS, Dúbravská cesta 9, SK-84506 Bratislava, Slovakia |
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Abstract: | Five larval diets for laboratory rearing of Ceratitis capitata were tested. These diets were based on wheat bran, microcellulose, potato starch and agar. To evaluate the quality of diet,
pupal rearing efficacy and pupal weight were checked. The best results were obtained with an agar based diet used for Manduca sexta laboratory rearing. To simplify the preparation and to reduce the cost of this diet, a new formulation was developed. Larvae
reared on the new agar-based diet achieved higher pupal rearing efficacy than larvae reared on bran diet recently used in
medfly mass rearing facilities. Heat treated medflies reared on the new agar-based diet achieved similar pupal rearing efficacy
with heat treated medflies reared on bran diet. When testing population density, higher pupal rearing efficacy was again achieved
on new diet. The highest pupal rearing efficacy was achieved with 100 eggs per 25 g of diet, lowest with 500 eggs per 25 g
of diet. Concerning pupal weight, there was no difference in results achieved on Petri dishes with different larval population
densities. Larvae reared on new agar-based diet reached better results than larvae reared on bran diet. The preparation of
the new diet is simple and the cost is low, so it is good for laboratory tests and rearing. |
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Keywords: | Ceratitis capitata larval diet laboratory rearing bran agar |
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