A method for harvesting non-cultivable filamentous segmented microbes inhabiting the ileum of mice |
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| Authors: | GW Tannock CM Crichton DC Savage |
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| Institution: | Department of Microbiology, University of Otago, Dunedin, New Zealand;Department of Microbiology, University of Illinois, Urbana, IL, U.S.A. |
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| Abstract: | A method by which non-cultivable filamentous segmented microbes colonising the ileal epithelium of mice can be harvested was devised. Intact epithelial cells with attached filamentous microbes were obtained. An ethanol-treated preparation of epithelial material was used to restore the filamentous segmented microbes to the normal microflora of mice maintained by gnotobiotic methodology. |
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| Keywords: | Epithelium Discontinuous density gradient centrifugation |
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