NbLRK1, a lectin-like receptor kinase protein of <Emphasis Type="Italic">Nicotiana benthamiana</Emphasis>, interacts with <Emphasis Type="Italic">Phytophthora infestans</Emphasis> INF1 elicitin and mediates INF1-induced cell death |
| |
Authors: | Hiroyuki Kanzaki Hiromasa Saitoh Yoshihiro Takahashi Thomas Berberich Akiko Ito Sophien Kamoun Ryohei Terauchi |
| |
Institution: | (1) Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami Iwate, 024-0003, Japan;(2) The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8, Ueda, Morioka Iwate, 020-8550, Japan;(3) The Sainsbury Laboratory, John Innes Centre, Norwich, UK |
| |
Abstract: | Phytophthora infestans INF1 elicitin causes the hypersensitive response (HR) in Nicotiana benthamiana (Kamoun et al. in Plant Cell 10:1413–1425, 1998). To identify N. benthamiana proteins that interact with INF1, we carried out a yeast two-hybrid screen. This screen resulted in the isolation of a gene
NbLRK1 coding for a novel lectin-like receptor kinase. NbLRK1 interacted with INF1 through its VIb kinase subdomain. Purified INF1
and NbLRK1 proteins also interacted in vitro. INF1 treatment of N. benthamiana leaves induced autophosphorylation of NbLRK1. Most importantly, virus-induced gene silencing (VIGS) of NbLRK1 delayed INF1-mediated HR in N. benthamiana. These data suggest that NbLRK1 is a component of the N. benthamiana protein complex that recognizes INF1 elicitor and transduces the HR signal. |
| |
Keywords: | Autophosphorylation Hypersensitive response INF1 NbLRK1 Nicotiana Virus-induced gene silencing Y2H |
本文献已被 PubMed SpringerLink 等数据库收录! |
|