Examples of expression systems based on animal RNA viruses: alphaviruses and influenza virus. |
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| Authors: | C M Rice |
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| Affiliation: | Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110-1093. |
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| Abstract: | Successful recovery of RNA viruses and functional RNA replicons from cDNA has greatly facilitated molecular genetic analyses of viral proteins and cis-regulatory elements. This technology allows the use of RNA virus replication machinery to express heterologous sequences. Both positive-strand and negative-strand animal RNA viruses have been engineered to produce chimeric viruses expressing protective epitopes from other pathogens and for transient expression of heterologous sequences. |
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