| 紫外分光光度法测定木棉叶中总黄酮的含量 |
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| 引用本文： | 刘欣欣,王国凯,秦民坚.紫外分光光度法测定木棉叶中总黄酮的含量[J].中国野生植物资源,2011,30(6):70-72. |
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| 作者姓名： | 刘欣欣 王国凯 秦民坚 |
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| 作者单位： | 中国药科大学中药资源学研究室,江苏南京211198;中国药科大学教育部现代中药重点实验室,江苏南京211109 |
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| 基金项目： | 国家自然科学基金项目资助 |
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| 摘 要： | 目的:测定木棉叶中总黄酮的含量.方法:采用紫外分光光度法在258 nm处测定吸光度.结果:对照品溶液在1.32～13.2 μg/mL范围内线性关系良好(r =0.9996),平均加样回收率为100.83％,RSD =3.13％.结论:该方法简便、灵敏、准确,可用于木棉叶的质量控制.
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| 关 键 词： | 木棉叶 总黄酮 紫外分光光度法 |
Determination of Total Flavonoids in Leaves of Bombax ceiba L.by UV Spectrophotometry |
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| Liu XinXin,Wang Guokai,Qin Minjian.Determination of Total Flavonoids in Leaves of Bombax ceiba L.by UV Spectrophotometry[J].Chinese Wild Plant Resources,2011,30(6):70-72. |
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| Authors: | Liu XinXin Wang Guokai Qin Minjian |
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| Institution: | 1. Department of Resources Science of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 211198, China; 2. Key Laboratory of Modem Traditional Chinese Medicines of Ministry of Education,China Pharmaceutical University, Nanjing 210009, China) |
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| Abstract: | Objective： Determine the contents of total flavnoids in leaves of Bombax ceiba L. Method： Determine the absorbance at the wavelength of 258 nm by UV spectrophotometry. Result：Linear relationship was good in the range of 1.32 - 13.2 ug/mL （r =0.9996）, the average recoveries was 100.83 %, RSD =3.13 %. Conclusion： The method is simple, sensitive, accurate, and could be used to control the quality of leaves of Bombax ceiba L. |
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| Keywords: | Bombax ceiba L total flavonoids UV spectrophotometry |
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