| HPLC法同时测定千斤拔属植物7种黄酮的含量 |
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| 引用本文： | 李莉,刘志华,秦民坚. HPLC法同时测定千斤拔属植物7种黄酮的含量[J]. 中国野生植物资源, 2011, 30(5): 54-57,65. DOI: 10.3969/j.issn.1006-9690.2011.05.013 |
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| 作者姓名： | 李莉 刘志华 秦民坚 |
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| 作者单位： | 1. 中国药科大学中药资源学研究室,江苏南京210009;中国药科大学教育部现代中药研究重点实验室,江苏南京210009
2. 中国医学科学北京协和研究院药用植物研究所,北京,100193 |
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| 摘 要： | 目的:测定千斤拔属7种植物中的7种黄酮类的含量,为千斤拔类药材质量控制提供依据。方法:色谱条件为Hedera C18色谱柱(250 mm×4.6 mm,5.0μm):流速1.2 mL/min,流动相为0.5%HAc水和乙腈梯度洗脱,柱温35℃,检测波长263 nm。结果:七种黄酮类成分在90 min内达到现行分离,线性关系良好(,R2>0.9995),日内、日间精密度、稳定性和重复性的RSD≤3.0%。该法成功的应用于千斤拔药材7种黄酮的评均回收率分别为96.94%～105.29%(RSD≤2.98%)结论:本方法准确、快速、重现性好,不同品种的千斤拔药材质量存在差异,可以为研究千斤拔药材的质量标准提供依据。
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| 关 键 词： | 千斤拔 HPLC 黄酮 含量测定 |
Simultaneous Determination of Seven Flavonoids in the Radix of Flemingia by HPLC |
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| Li Li,Liu Zhihua,Qin Minjian. Simultaneous Determination of Seven Flavonoids in the Radix of Flemingia by HPLC[J]. Chinese Wild Plant Resources, 2011, 30(5): 54-57,65. DOI: 10.3969/j.issn.1006-9690.2011.05.013 |
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| Authors: | Li Li Liu Zhihua Qin Minjian |
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| Affiliation: | Li Li1,2,Liu Zhihua3,Qin Minjian1,2 [1.Department of Resources Science of Traditional Chinese Medicines,China Pharmaceutical University,Nanjing 210009,China,2.Key Laboratory of Modern Traditional Chinese Medicines(Ministry of Education),3.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193] |
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| Abstract: | Objective： To develop a High-Performance Liquid Chromatography（HPLC） method for the simultaneous determination of the radix of Flemingia.Method： The separation was carried out on a Hedera ODS（4.6 mm×250 mm,5 μm） column eluted with in mobile phases of water containing 0.5% HAc and Acetonitrile gradient mode at the flow rate of 1.2 mL/min.The detective wavelength was 263nm and column temperature was 35 ℃.Results： The good separation of seven flavonoids was achieved within 90 min and showed good linear relationship（R20.9995）,with the RSD of intra-day and inter-day presision,repeatability and stability3.0%.The average recoveries of the seven flavonids were within 96.94%-105.29%（RSD≤2.98%）.Conculsion： The developed method is simple,accurate and repeatable,and can be readily used as a powerful tool for the quality control of Flemingia. |
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| Keywords: | Flemingia HPLC flavonoids determination |
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