Characterization and expression analysis of a trypsin-like serine protease from planarian <Emphasis Type="Italic">Dugesia japonica</Emphasis> |
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Authors: | Luming Zhou Suge Wu Dianchen Liu Bo Xu Xiufang Zhang Bosheng Zhao |
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Institution: | (1) Laboratory of Developmental and Evolutionary, School of Life Sciences, Shandong University of Technology, 12 Zhangzhou Road, Zibo, 255049, People’s Republic of China;(2) School of Life Sciences, Yantai University, Yantai, 264005, People’s Republic of China; |
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Abstract: | Trypsin-like serine proteases are involved in large number of processes, especially in digestive degradation and immune responses.
Here, we identify the characterization of a trypsin-like serine protease in planarian, Djtry, which interestingly has the incompletely conserved catalytic triad (K, D, and S). Phylogenetic analysis suggests that Djtry
is an ancient type of trypsin-like serine proteases. The spatial and temporal expression patterns of Djtry are shown during regenerating and embryonic development by whole-mount in situ hybridization. Djtry is found to display a tissue specific expression pattern, with a predominant expression detected in whole gut region of intact
and regenerating planarian. While the tissue- and stage-specific expression patterns during the embryonic development imply
the roles of Djtry involve in yolk degradation and gut formation. Quantitative real-time PCR was carried out to analyze the
function of this protease in vivo after planarians were stimulated to a bacterial challenge and food. The results showed that
Djtry increased after a bacterial challenge and was basically stable for food. Therefore, the trypsin-like serine protease might
be involved in the innate defense reactions against bacterial infection. |
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