陆地棉优质纤维渐渗系中外源遗传组分的鉴定与分析

鲁原343是一个渐渗了海岛棉优质纤维基因的陆地棉种质,对其渐渗的优质纤维片段进行鉴定,对利用优质纤维渐渗系改良陆地棉品种的纤维品质具有重要意义。本研究以综合性状优良的转基因抗虫棉鲁棉研22号与鲁原343杂交构建作图群体,利用317对SSR引物对鲁原343和鲁棉研22号进行多态性分析,有24对引物表现多态。利用这些引物进一步和TM-1、优质纤维渐渗片段的供体Ash imoun i作比较,初步鉴定出10个SSR位点与海岛棉渐渗有关。利用这些标记分析(鲁棉研22×鲁原343)F2群体的标记基因型和纤维品质性状的关系,6个标记与纤维品质显著相关,涉及到4条染色体。其中与伸长率相关的标记BNL2986(R2=5.87%)和与长度、细度相关的标记NAU751(R2=6.62%,6.01%)同位于16号染色体的连锁群LG1上,标记间距离为17.7 cM;与纤维成熟度相关的标记BNL3590(R2=8.62%)和与成熟度、伸长率相关的标记BNL3971(R2=15.0%,9.79%)位于2号染色体的连锁群LG3上,标记间距离为4.5 cM;与纤维强度相关的标记BNL3279(R2=8.12%)和与细度相关的标记BNL827(R2=13.94%)分别位于LGD02和25号染色体上。

Identification of Exotic Genetic Components of Upland Cotton (Gossypium hirsutum L.) Introgression Line with High Fiber Qualities from Sea-island Cotton (Gossypium barbadense L.)

Genetic population of F2 and its corresponding F2:3 lines derived from a hybridization combination of Lumianyan22 and Luyuan 343 were used to evaluate the fiber quality and detect the related introgression genes by SSR markers.10 SSR loci were proved to be derived from the Island cotton primogenitor by compared all polymorphic markers.10 SSR loci were proved to be derived from the Island cotton primogenitor by compared all polymorphic marker loci with the Island cotton primogenitor(Ashimouni,a famous Egypt Island cotton cultivar) and the standard genetic line(TM-1) of Upland cotton.There were at least 6 marker loci,which involved in 4 chromosomes,significantly related to different parameters of high fiber quality in this introgression upland cotton germplasm.Statistical analysis and linkage analysis based on the same population showed that,marker loci BNL2986 which related to fiber elongation(R2=5.87%) and NAU751 which related to fiber length(R2=6.62%) and fiber fineness(R2=6.01%) were linked with 17.7cM of genetic distance and located on Chromosome 16.BNL3590 which related to fiber maturation(R2=8.62%) and BNL3971 which related to fiber maturation(R2=15.0%) and fiber elongation(R2=9.79 %) were linked with 4.5 cM and located on Chromosome 2.While BNL3279 which related to fiber strength(R2=8.12%) and BNL827 that related to fiber fineness(R2=13.94%) were dispersed in LGD02 and chromosome 25 respectively.