首页 | 本学科首页   官方微博 | 高级检索  
   检索      


A selective λ phage cloning vector with automatic excision of the insert in a plasmid
Authors:Ichiro N Maruyama  Sydney Brenner  
Institution:

MRC Molecular Genetics Unit, Hills Road, Cambridge CB2 2QH, UK

Abstract:A bacteriophage λ cloning vehicle has been constructed for the generation of cDNA libraries. The vector has the following properties. (1) It has a unique BamHI site engineered into the λ gam gene. Segments of DNA can be cloned into this site and clones with an insert can be selected by their ability to grow on an Escherichia coli host lysogenic for phage P2 (Spi? phenotype). (2) When the recombinant phage infects a Cre-producing E. coli strain, a site-specific recombination event results in the excision of a plasmid replicon with the cloned insert. (3) Single-stranded DNAs can be recovered by growing helper M13 phages on bacteria harboring such plasmids. The vector, λMGU2, has been used to construct a nematode (Caenorhabditis elegans) cDNA library.
Keywords:Spi selection  Caenorhabditis elegans  cDNA library  bacteriophage P1  cre-lox site-specific recombination  phagemid
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号