Voltammetric detection of trimethylamine using immobilized trimethylamine dehydrogenase on an electrodeposited goldnanoparticle electrode |
| |
Authors: | Young-Bong Choi Hee Gon Kim Gui Hwan Han Hyug-Han Kim Si Wouk Kim |
| |
Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain; |
| |
Abstract: | A voltammetric enzyme electrode was developed based on nicotinamide-independent trimethylamine dehydrogenase (TMADH, EC 1.5.99.7),
which catalyses the oxidation of trimethylamine (TMA) to dimethylamine and formaldehyde. A quaternized osmium hydrogel polymer,
poly(vinylimidazole-Os(4,4′-dimethyl-2,2′-bipyridine)2Cl]+/2+) with ethylamine (PVI-Os-EA), was prepared as a potential redox mediator in an electrochemical biosensor. TMA was detected
using TMADH that was co-immobilized with an osmium hydrogel polymer on electrodeposited gold nanoparticles (Au-NPs) on screen-printed
carbon electrodes (SPCEs). The Au-NPs deposited onto SPCEs provided about a three times higher electrochemical response compared
to that of a planar gold electrode. As TMA was catalyzed by wired TMADH, the electrical signal was monitored at 0.3 V versus
Ag/AgCl by cyclic voltammetry and chronoamperometry. The anode currents increased linearly in proportion to the TMA concentration
over the 0 ∼ 2.5 mM range with a detection limit of 1 μM (R = 0.9972). |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|