A novel thermostable and halostable carboxymethylcellulase from marine bacterium Bacillus licheniformisAU01 |
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Authors: | N. Annamalai R. Thavasi S. Vijayalakshmi T. Balasubramanian |
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Affiliation: | (1) CAS in Marine Biology, Annamalai University, Parangipettai, 608502, Tamilnadu, India;(2) Department of Chemical and Biological Sciences, Polytechnic Institute of New York University, Six Metrotech Center, Brooklyn, 11201, NY, USA |
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Abstract: | A novel thermostable, halostable carboxymethylcellulase (CMCase) from a marine bacterium Bacillus licheniformisAU01 was purified 10.4-fold with 18% yield with a specific activity of 88.43 U/mg and the molecular weight was estimated as 37 kDa. The enzyme was optimally active at pH 9–10 and temperature 50–60°C and it was most stable up to pH 12 and 20–30% of NaCl concentration. The enzyme activity was reduced when treated with Hg2+, Fe2+ and EDTA and stimulated by Co2+, Mn2+, Mg2+ and Ca2+. Various cationic, anionic detergents and commercial detergents were not much affected CMCase activity. |
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