抑制Runx2的表达增强BMP2诱导的干细胞成软骨分化

Suppression of Runx2 Potentiates BMP2-induced Chondrogenic Differentiation

Objective: To investigate the potentiation effect of RNA silenced Runx2 on bone morphogenetic protein 2 (BMP2) induced chondrogenic differentiation of mesenchymal stem cells (MSCs). Method: C3H10T1/2 cells were treated with adenovirus: Ad-BMP2, Ad-SiRunx2 or Ad-GFP. The sulfated glycosaminoglycan of cellular matrix was detected by Alcian Blue Staining. The mRNA level of Runx2, Col2a1, aggrecan , and Col10a1 were detected by RT-PCR,and the protein level of BMP2, Col2a1,Col10a1 were detected by Western blot .Results: The BMP2+SiRunx2 group (C3H10T1/2 cells were treated with AdBMP2 and AdSiRunx2) was more blue than the BMP2 group on day 10 proven by Alcian blue staining. The BMP2+SiRunx2 group showed a higher mRNA level of Col2a1 and aggrecan (P<0.05) and a lower protein level of Col10a1 (P<0.05) than the BMP2 group on day 7 and 14. The BMP2+SiRunx2 group showed a higher protein level of Col2a1(P<0.05) and a lower protein level of Col10a1(P<0.05) than the BMP2 group on day 10. Conclusion: The siRNA targeting Runx2 promotes BMP2-induced stem cell chondrogenic differentiation and supresses the chondrocyte hypertrophy and maturation.