| D-核糖生产菌的选育及发酵工艺的改进 |
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| 引用本文： | 夏海平,吴义明. D-核糖生产菌的选育及发酵工艺的改进[J]. 氨基酸和生物资源, 2006, 28(4): 30-32. DOI: 10.3969/j.issn.1006-8376.2006.04.008 |
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| 作者姓名： | 夏海平 吴义明 |
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| 作者单位： | 上海迪赛诺生物技术有限公司,上海,201302;上海迪赛诺生物技术有限公司,上海,201302 |
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| 摘 要： | 通过对菌株BX-2(Bacillus subtilis)的紫外诱变和硫酸二乙酯的诱变处理得到D-核糖高产菌株BY-5。36℃,pH6.8,250 r.m in-1条件下培养72 h,其D-核糖产量从开始的55.0 g.L-1提高到68.0 g.L-1。蔗糖和葡萄糖的混合碳源发酵,D-核糖产量达到75 g.L-1。当D-葡萄糖被蔗糖部分替代后,D-核糖生产能力得到了提高。
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| 关 键 词： | D-核糖 育种 混合碳源 |
| 文章编号： | 1006-8376(2006)04-0030-03 |
| 收稿时间： | 2006-07-02 |
| 修稿时间： | 2006-07-02 |
Breeding of A High- D- Ribose - Producing Strain and Improvement of Fermentation Technology |
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| XIA Hai-ping,WU Yi-ming. Breeding of A High- D- Ribose - Producing Strain and Improvement of Fermentation Technology[J]. Amino Acids & Biotic Resources, 2006, 28(4): 30-32. DOI: 10.3969/j.issn.1006-8376.2006.04.008 |
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| Authors: | XIA Hai-ping WU Yi-ming |
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| Abstract: | The strain BX-2(Bacillus subtilis) was matagenized by ultraviolet light and DES,a high-D-ribose-producing strain(BY-5) was obtained.Cultivated for 72h under the condition of 36℃,pH6.8 and 250r·min~(-1),the D-ribose content of the BY-5 strain was as high as 68.0g·L~(-1) from the original 55.0g·L~(-1).Fermentation with mixed carbon sources of glucose and sucrose could make the yield of D-ribose as high as 75g·L~(-1).When D-glucose was quantitatively replaced by sucrose the D-ribose production was enhenced. |
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| Keywords: | D-ribose breeding mixed carbon sources |
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