| 大豆基因型对根癌农杆菌菌株敏感性的研究 |
| |
| 引用本文： | 王罡,王萍,蔺宇,张领兵,吴颖.大豆基因型对根癌农杆菌菌株敏感性的研究[J].遗传,2002,24(3):297-300. |
| |
| 作者姓名： | 王罡 王萍 蔺宇 张领兵 吴颖 |
| |
| 作者单位： | 解放军军需大学植物基因工程研究中心，植物分子生物学研究室,长春 130062
Laboratory of Plant Moleculal Biology,Research Center For Plant Genetic Engineering,
The Quatermaster University of PLA,Changchun 130062,China |
| |
| 基金项目： | 国家植物转基因中试及产业化基地专项基金 (项目编号J99-B -0 0 1) |
| |
| 摘 要： | 以栽培大豆［Glycine max (L.) Mer］吉林30、吉林43、绥农8、黑农35和东农42等的下胚轴为外植体，用EHA105和LBA4404 2个根癌农杆菌菌株（分别含有pGBI121S4ABC和pGBI4A2B质粒）研究大豆基因型对根癌农杆菌的敏感性，以及根癌农杆菌对大豆的侵染能力。结果表明，大豆基因型对根癌农杆菌的敏感性存在显著差异，以吉林43最敏感。根癌农杆菌菌株对大豆下胚轴侵染能力不同，含有pGBI121S4ABC质粒的LBA4404侵染能力较强，但差异未达显著水平。
Abstract:The sensitivity of genotypes in soybean to lines of Agrobacterium tumefaciens and the ability of A.tumefaciens infecting to soybean were investigated with hypocotyls of soybean (Jilin30,Jilin43,Suinong8,Heinong35 and Dongnong42) and lines of A.tumefaciens LBA4404 and EHA105 which including plasmid pGBI121S4ABC and pGBI4A2B respectively.The results showed that the sensitivity of genotypes in soybean to A.tumefaciens was significantly different.Jilin43 was the most sensitive materials to A.tumefaciens.The ability of A.tumefaciens infecting hypocotyls in soybean was different.LBA4404 including plasmid pGBI121S4ABC was easier to infect hypocotyls of soybean.
|
| 关 键 词： | 根癌农杆菌 下胚轴 Key words soybean 基因型 大豆 |
| 文章编号： | 0253-9772(2002)03-0297-04 |
| 修稿时间： | 2001年11月12 |
The Studies of Sensitivity of Genotypes in Soybean to Lines of Agrobacterium tumefaciens |
| |
| WANG Gang,WANG Ping,LIN Yu,ZHANG Ling bing,WU Ying.The Studies of Sensitivity of Genotypes in Soybean to Lines of Agrobacterium tumefaciens[J].Hereditas,2002,24(3):297-300. |
| |
| Authors: | WANG Gang WANG Ping LIN Yu ZHANG Ling bing WU Ying |
| |
| Institution: | Laboratory of Plant Moleculal Biology, Research Center For Plant Genetic Engineering, The Quatermaster University of PLA, Changchun 130062, China. |
| |
| Abstract: | The sensitivity of genotypes in soybean to lines of Agrobacterium tumefaciens and the ability of A.tumefaciens infecting to soybean were investigated with hypocotyls of soybean (Jilin30,Jilin43,Suinong8,Heinong35 and Dongnong42) and lines of A.tumefaciens LBA4404 and EHA105 which including plasmid pGBI121S4ABC and pGBI4A2B respectively.The results showed that the sensitivity of genotypes in soybean to A.tumefaciens was significantly different.Jilin43 was the most sensitive materials to A.tumefaciens .The ability of A.tumefaciens infecting hypocotyls in soybean was different.LBA4404 including plasmid pGBI121S4ABC was easier to infect hypocotyls of soybean. |
| |
| Keywords: | soybean genotypes Agrobacterium tumefaciens hypocotyl |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
