Improved single-chain transactivators of the Tet-On gene expression system |
| |
| Authors: | Xue Zhou Jori Symons Rieuwert Hoppes Christel Krueger Christian Berens Wolfgang Hillen Ben Berkhout Atze T Das |
| |
| Institution: | (1) Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Meibergdreef 15, 1105, AZ, Amsterdam, The Netherlands;(2) Lehrstuhl fur Mikrobiologie, Institut fur Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Staudtstrasse 5, D-91058 Erlangen, Germany |
| |
| Abstract: | Background The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems
are based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding protein that binds to specific operator sequences (tetO). To allow the independent regulation of multiple genes, novel Tet systems are being developed that respond to different
effectors and bind to different tetO sites. To prevent heterodimerization when multiple Tet systems are expressed in the same cell, single-chain variants of the
transactivators have been constructed. Unfortunately, the activity of the single-chain rtTA (sc-rtTA) is reduced when compared
with the regular rtTA, which might limit its application. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
