Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains |
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| Authors: | Aurelien Olichon Daniel Schweizer Serge Muyldermans Ario de Marco |
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| Institution: | (1) Cell Biology Unit, EMBL – Meyerhofstr. 1, D-69117 Heidelberg, Germany;(2) Scientific Core Facilities, EMBL – Meyerhofstr. 1, D-69117 Heidelberg, Germany;(3) Department of Cellular and Molecular Interactions, VIB, Vrije Unversiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium;(4) IFOM-IEO Campus, Biochemistry Unit, via Adamello 16, I-20139 Milano, Italy |
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| Abstract: | Background Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications
because of their small size, robustness, easy handling and possibility to refold after chemio-physical denaturation. Their
heat tolerance is a particularly interesting feature because it has been recently related to both high yields during recombinant
expression and selective purification of folded protein. |
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