Quantitative determination by real-time PCR of four vaginalLactobacillus species,Gardnerella vaginalisandAtopobium vaginaeindicates an inverse relationship betweenL. gasseriandL. iners |
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| Authors: | Ellen De Backer Rita Verhelst Hans Verstraelen Mohammed A Alqumber Jeremy P Burton John R Tagg Marleen Temmerman and Mario Vaneechoutte |
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| Institution: | (1) Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, UGent, Ghent, Belgium;(2) Department of Obstetrics & Gynaecology, Ghent University Hospital, UGent, Ghent, Belgium;(3) Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand;(4) BLIS Technologies Ltd., Centre for Innovation, University of Otago, Dunedin, New Zealand |
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| Abstract: | Background Most studies of the vaginal microflora have been based on culture or on qualitative molecular techniques. Here we applied
existing real-time PCR formats forLactobacillus crispatus,L. gasseriandGardnerella vaginalisand developed new formats forAtopobium vaginae,L. inersandL. jenseniito obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant
women aged between 18 and 45 years. |
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