| 棕色固氮菌钼铁蛋白结晶及一些物理化学性质 |
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| 引用本文： | 王业勤,戴玲芳,何家苑,杜代贤,林惠民,何振荣,李辛夫,谭渝云,黎尚豪.棕色固氮菌钼铁蛋白结晶及一些物理化学性质[J].水生生物学报,1980,4(1):67-74. |
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| 作者姓名： | 王业勤 戴玲芳 何家苑 杜代贤 林惠民 何振荣 李辛夫 谭渝云 黎尚豪 |
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| 作者单位： | 中国科学院水生生物研究所第五室生化、遗传组
(王业勤，戴玲芳，何家苑，杜代贤，林惠民，何振荣，李辛夫，谭渝云)，中国科学院水生生物研究所第五室生化、遗传组(黎尚豪) |
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| 摘 要： | 获得了棕色固氮菌固氮酶钼铁蛋白的针状结晶,大小为2-2.5×25-65微米,成晶所要求的条件不是很严格的。SDS 电泳和氨基酸分析结果表明,钼铁蛋白分子量为250,000。只观察到一种类型亚单位。其中酸性氨基酸占优势。分子氧使针状晶体演变为发状晶丝,晶丝又密集成束。晶束可保存较长时间。氧使钼铁蛋白在电泳时形成高聚合态。
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CRYSTALLIZATION AND SOME PHYSICOCHEMICAL PROPERTIES OF MO-FE PROTEIN OF AZOTOBACTER VINELANDII NITROGENASE |
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{{/each}}.CRYSTALLIZATION AND SOME PHYSICOCHEMICAL PROPERTIES OF MO-FE PROTEIN OF AZOTOBACTER VINELANDII NITROGENASE[J].Acta Hydrobiologica Sinica,1980,4(1):67-74. |
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| Abstract: | A series of experiments were carried out to study the crystallizing conditions of Mo-Fe protein of Azotobacter vinclandii nitrogenase. Needle-like crystals of Mo-Fe protein were obtained, with crystal size fluctuated inthe range of 2-2.5×25-65μ. The conditions required for crystallization were notvery strict. The molecular weight of Mo-Fe protein was estimated by SDS gel electrophoresisand by amino acid analysis to be approximately 250000. Only one type of subunit wasobserved; in the Mo-Fe protein acidic amino acid was predominant. It was found that oxygen caused the needle-like crystals to transform into hair-like crystals which may aggregate somewhere further into bundles. The latter underanaerobic condition could last for longer time. It was also demonstrated that duringthe gel electrophoresis the formation of a higher molecular weight polymer was depend-ent on the presence of oxygen.
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