Spontaneous Release of γ-Aminobutyric Acid Formed from Putrescine and Its Enhanced Ca2+-Dependent Release by High K+ Stimulation in the Brains of Freely Moving Rats |
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| Authors: | Tadashi Noto Hiromichi Hashimoto Junji Nakao Hiroshi Kamimura Teruo Nakajima |
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| Institution: | Department of Psychiatry, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, Japan |
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| Abstract: | The spontaneous release of 3H] gamma-aminobutyric acid (3H]GABA) in various areas of rat brain injected with 3H]putrescine was examined using a push-pull perfusion technique. The release in a 25-min perfusate was highest in the caudate-putamen. The effect of high K+ stimulation on the release of 3H]GABA formed from 3H]putrescine was examined in the caudate-putamen. The release was enhanced by high K+ solution in a Ca2+-dependent manner. |
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| Keywords: | γ-Aminobutyric acid release Putrescine K+ stimulation Push-pull perfusion |
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