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Streptomyces sp. Z-11-6--a new producer of extracellular L-glutamate oxidase
Authors:Sukhacheva M V  Netrusov A I
Institution:Department of Microbiology, Biological Faculty, Moscow State University, Vorob'evy gory, Russia.
Abstract:Glutamate oxidase activity was studied in 1254 Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis were optimized. A multistep-mutagenesis and selection procedure allowed a genetically stable strain, Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate.
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