Proline 78 is crucial for human immunodeficiency virus type 1 Nef to down-regulate class I human leukocyte antigen |
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Authors: | Yamada Takeshi Kaji Naotoshi Odawara Takashi Chiba Joe Iwamoto Aikichi Kitamura Yoshihiro |
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Affiliation: | Division of Infectious Diseases, Advanced Clinical Research Center, The University of Tokyo, Japan. |
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Abstract: | Human immunodeficiency virus type 1 Nef down-regulates human leukocyte antigen class I (HLA-I) in T lymphocytes, and the down-regulation involves the Nef proline-rich domain (PRD) containing four prolines at positions 69, 72, 75, and 78. We used a Sendai virus vector with nef and examined regulation by Nef of HLA-I and CD4 in suspension cultures of cells such as T lymphocytes. Analyses of a series of PRD substitution mutants indicated that, because the substitution of Pro78 with Ala abolished down-regulation of HLA-I but not of CD4, Pro78 is important for HLA-I down-regulation in T lymphocytes. |
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