Sodium gluconate production byAspergillus niger with intermittent broth replacement |
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Authors: | Sang-Yoon Lee Bu-Su Park Jin-Hyup Kim Byung-Gee Kim Dong-Il Kim |
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Institution: | (1) Department of Biological Engineering, Inha University, 402-751 Inchon, Korea;(2) Alchun Industry Co. Ltd., 325-800 Seochun, Korea;(3) Division of Chemical Engineering, Seoul National University, 151-742 Seoul, Korea |
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Abstract: | Intermittent broth replacement was carried out to enhance the productivity and purity of sodium gluconate usingAspergillus niger by reducing the concentration of unmetabolized glucose. As inoculum size increased, length of lag phase was shortened and
high initial production rate of sodium gluconate was achieved. However, too high inoculum concentration lowered productivity
during the later stage of fermentation and increased residual glucose at the end of cultivation. When culture broth was replaced
intermittently with distilled water, fresh medium, or recycled medium for comparison with traditional fermentation method,
production of sodium gluconate was enhanced more than 1.5 fold and active production period could be prolonged without residual
glucose. In addition, productivity was maintained at a level higher than 6 g/L/nr. Therefore, it was found that the reduction
of biomass and viscosity by intermittent broth replacement could enhance the productivity. |
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Keywords: | gluconic acid sodium gluconate intermittent broth replacement Aspergillus niger |
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