SSR allelic variation in almond (Prunus dulcis Mill.) |
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Authors: | Hua Xie Yi Sui Feng-Qi Chang Yong Xu Rong-Cai Ma |
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Institution: | (1) Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Ban-Jing Rd., Hai-Dian District, 100089 Beijing, China;(2) College of Life Sciences, Capital Normal University, 100037 Beijing, China |
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Abstract: | Sixteen SSR markers including eight EST-SSR and eight genomic SSRs were used for genetic diversity analysis of 23 Chinese
and 15 international almond cultivars. EST- and genomic SSR markers previously reported in species of Prunus, mainly peach, proved to be useful for almond genetic analysis. DNA sequences of 117 alleles of six of the 16 SSR loci were
analysed to reveal sequence variation among the 38 almond accessions. For the four SSR loci with AG/CT repeats, no insertions
or deletions were observed in the flanking regions of the 98 alleles sequenced. Allelic size variation of these loci resulted
exclusively from differences in the structures of repeat motifs, which involved interruptions or occurrences of new motif
repeats in addition to varying number of AG/CT repeats. Some alleles had a high number of uninterrupted repeat motifs, indicating
that SSR mutational patterns differ among alleles at a given SSR locus within the almond species. Allelic homoplasy was observed
in the SSR loci because of base substitutions, interruptions or compound repeat motifs. Substitutions in the repeat regions
were found at two SSR loci, suggesting that point mutations operate on SSRs and hinder the further SSR expansion by introducing
repeat interruptions to stabilize SSR loci. Furthermore, it was shown that some potential point mutations in the flanking
regions are linked with new SSR repeat motif variation in almond and peach.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. |
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