Cytoplasmic events in human meiotic arrest as revealed by immunolabelling of spermatocyte proacrosin |
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Authors: | Denise Escalier,Diego Bermù dez,Jean-Marc Gallo ,Annick Viellefond,Joseph Schré vel |
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Affiliation: | Laboratoire de Biologie de la Reproduction et du Développement, CHU Bicêtre, 78 Rue du Général Leclerc, F-94275 Le Kremlin Bicêtre, France;Departamento de Morfologia Normal y Patologica, Facultad de Medicina, Universidad de Malaga, E-29080 Malaga, Espana;Museum National d'Histoire Naturelle and UA CNRS 114, 61 Rue Buffon, F-75231 Paris Cedex 05, France;Département d'Anatomo-Pathologie, CHU Bicêtre, 78 Rue du Général Leclerc, F-94275 Le Kremlin Bicêtre, France |
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Abstract: | Abstract. Proacrosin appears in the Golgi complex as early as the mid-pachytene stage and immediately thereafter initiates partition to be equally distributed in sper-matids. The anti-proacrosin monoclonal antibody 4D4 (mAb 4D4) was used as a marker of these cytoplasmic events in ten men exhibiting spermatogenesis arrest in three specific stages: (i) leptotene/zygotene spermatocyte I with impaired chromosome pairing (six cases), (ii) early pachytene I (one case) and (iii) metaphase/anaphase I (three cases). Prophase arrest stages were identified on testis sections stained by silver nitrate. MAb 4D4 labelling revealed two types of leptotene/zygotene arrest depending on whether proacrosin was expressed or not. The data obtained enabled us to distinguish between: (i) nuclear blockages due to chromosome and/or nuclear matrix anomalies, when cytoplasmic events were either inhibited or not inhibited, and (ii) nuclear anomalies due to microtubular disturbances. In this latter case, cytokinesis was impaired as early as the prophase I, thus indicating a relationship between the Golgi partitioning and the microtubule network. Data show that meiotic arrest investigations, by means of an appropriate marker of the cytoplasmic events, provide valuable information on spermatogenic developmental processes. |
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Keywords: | Acrosome Meiosis Monoclonal antibody Proacrosin Spermatocyte Human spermatogenesis |
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