Collection method, season and individual variation on seminal characteristics in the llama (Lama glama) |
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| Authors: | Giuliano S Director A Gambarotta M Trasorras V Miragaya M |
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| Institution: | Areas de Física Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Chorroarín 280, (1427) Ciudad Autónoma de Buenos Aires, Argentina. smgiulia@fvet.uba.ar |
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| Abstract: | The objective of the present study was to evaluate the effect of semen collection method (electroejaculation "EE" as compared with the artificial vagina "AV"), the season (summer versus winter) and the male used on macroscopic and microscopic characteristics of ejaculates in llamas. A total of 110 ejaculates were collected from six males and 92 of them were analyzed. Ejaculate volume, concentration, total sperm and the following sperm characteristics were studied: motility, membrane function (HOS test), membrane integrity (CFDA/PI fluorochromes) and morphology. A mixed linear model, that considered season and collection method as the fixed variables and the male as the random variable, was used for the statistical analysis. Variability was found between males (p
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