In nature dormant buds and in vitro dormant-like buds ofFraxinus excelsior L. |
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Authors: | A Nougarède C E Silveira P Rondet |
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Institution: | (1) Laboratoire de Cytologie Expérimentale et Morphogenèse Végétale, Bâtiment N2, Case courrier 150, Université Pierre et Marie Curie, 4 place Jussieu, F-75252 Paris Cedex 05, France |
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Abstract: | Summary Microcuttings ofFraxinus excelsior, sampled from adult trees during the period of cell cycle blockage of bud in G0–1, developed long rejuvenated sprouts on the Woody Plant Medium (WPM) with benzylaminopurine (BAP) (4.0 mg/l) and indolebutyric acid (IBA) (0.03 mg/l). These sprouts had the ability to enter a resting period, building dormant-like buds when maintained on the original WPM. Sprouts developed from subcultures also entered a resting period without any transfer. Comparison of in nature buds in active growth and dormancy with buds of growing sprouts and in vitro dormant-like buds revealed similarity in behaviour at the shoot apical level. In particular, in dormant-like buds in a constant environment, shoot apical functioning was suppressed while the cell cycle of the shoot apex was blocked at the G0–1 phase, like in nature dormant buds.Abbreviations a.u.
arbitrary unit
- BAP
benzylaminopurine
- CF
cumulative frequency
- d1, d2
diameters of the shoot apex
- IBA
indolebutyric acid
- Pn
last opposite primordia of range n
- WPM
woody plant medium |
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Keywords: | Micropropagation Fraxinus excelsior Bud behaviour Cell cycle parameters Resting period |
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