Osmium ammine-B and electron spectroscopic imaging of ribonucleoproteins: Correlation of stain and phosphorus |
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Authors: | Ada L Olins Donald E Olins David P Bazett-Jones |
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Abstract: | Summary— Ultra-thin sections of Chironomus salivary glands were stained in a non-Feulgen procedure with osmium ammine-B and imaged at several electron energy-loss windows. For two types of RNP-containing structures (ie Balbiani ring granules and endoplasmic reticulum), a significant spatial correlation was observed between stain distribution and net phosphorus distribution. Non-Feulgen osmium ammine-B staining does not require the use of ultra-thin sections and can approximate the distribution of nucleic acid phosphorus. |
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Keywords: | osmium ammine-B energy filtered TEM RNA phosphorus mapping ribosome hnRNP Balbiani ring granules |
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