High resolution localization of cruzipain and Ssp4 in Trypanosoma cruzi by replica staining label fracture |
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Authors: | Alana E Nascimento Wanderley de Souza |
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Abstract: | Summary— The replica staining label fracture technique was used to analyse the distribution of cruzipain and Ssp4 in Trypanosoma cruzi. Intense labeling for the two proteins was seen on the E fracture face of amastigote forms. Gold particles did not co-localize with the intramembranous particles. Labeling was abolished by previous treatment of the parasites with phospholipase C from Trypanosoma brucei, which removes glycosylphosphatidyl inositol (GPI) anchored proteins. These observations suggest that cruzipain and Ssp4 are attached to the parasite surface via a GPI anchor. |
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Keywords: | Trypanosoma cruzi cruzipain replica staining |
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