Identification and Biological Activities of Long‐Chain Peptaibols Produced by a Marine‐Derived Strain of Trichoderma longibrachiatum |
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| Authors: | Mustapha Mohamed‐Benkada Yves François Pouchus Philippe Vérité Fabrice Pagniez Nathalie Caroff Nicolas Ruiz |
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| Institution: | 1. +213‐561‐677616;2. Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d'Oran‐Mohamed Boudiaf (USTO‐MB), Bir El Djir, Oran, Algeria;3. Faculté de Pharmacie, MMS, Université de Nantes, Nantes;4. Laboratoire de Chimie Analytique, Faculté de Médecine et Pharmacie, Université de Rouen, Rouen;5. Laboratoire de Parasitologie et Mycologie Médicale, IICiMed, Faculté de Pharmacie, Université de Nantes, Nantes;6. Laboratoire Thérapeutiques Cliniques et Expérimentales des Infections, Faculté de Médecine, Université de Nantes, Nantes |
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| Abstract: | Six long‐chain peptaibols, 1 – 6 , were identified from agar cultures of a marine‐derived Trichoderma longibrachiatum Rifai strain (MMS151) isolated from blue mussels. The structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (ESI‐IT‐MS) and GC/EI‐MS. The long‐chain peptaibols exhibited the general building scheme Ac‐Aib‐Ala‐Aib‐Ala‐Aib‐XXX‐Gln‐Aib‐Vxx‐Aib‐Gly‐XXX‐Aib‐Pro‐Vxx‐Aib‐XXX‐Gln‐Gln‐Pheol and were similar or identical to recurrent 20‐residue peptaibols produced by Trichoderma spp. Three new sequences were identified and were called longibrachins A‐0, A‐II‐a, and A‐IV‐b. The isolated peptaibols were assayed for cytotoxic, antibacterial, and antifungal activities, and acute toxicity on Dipteran larvae. |
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| Keywords: | Long‐chain peptaibols Marine‐derived fungi
Trichoderma longibrachiatum
Longibrachins A‐0 A‐II‐a and A‐IV‐b |
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