A localized linkage map of the citrus tristeza virus resistance gene region |
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Authors: | F. G. Gmitter Jr S. Y. Xiao S. Huang X. L. Hu S. M. Garnsey Z. Deng |
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Affiliation: | (1) Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 700 Experiment Station Road, 33850 Lake Alfred, FL, USA;(2) Huazhong Agricultural University, Citrus Research Institute, 430070 Wuhan, Hubei, PRC;(3) Yunnan Agricultural Extension Center, Kunming, Yunnan, PRC;(4) USDA/ARS, Horticultural Research Laboratory, 2120 Camden Road, 32803 Orlando, FL, USA |
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Abstract: | A localized genetic linkage map was developed of the region surrounding the citrus tristeza virus (CTV) resistance gene (designated Ctv) from Poncirus trifoliate L., a sexually compatible Citrus relative. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers in four intergeneric backcross families that were segregating for CTV resistance. Eight RAPD fragments were found that were consistently linked to Ctv in the four families. Map distances and locus order were determined with MAPMAKER 3.0, using the results obtained from 59 individuals in the largest family. Also, a consensus map was constructed with JOINMAP 1.3, using pooled results from the four backcross families. Marker orders were identical, except for 1 marker, on these independently developed maps. Family-specific resistance-associated markers were also identified, as were numerous susceptibility-associated markers. The identification of markers tightly linked to Ctv will enable citrus breeders to identify plants likely to be CTV-resistant by indirect, marker-assisted selection, rather than by labor-intensive direct challenge with the pathogen. These markers also provide a basis for future efforts to isolate Ctv for subsequent genetic manipulation.Florida Agricultural Experimental Station Journal Series No. R-04491 |
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Keywords: | Citrus L. Poncirus trifoliate L. Random amplified polymorphic DNA Bulked segregant analysis Genome mapping |
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