Size fractionation of DNA fragments by liquid-liquid chromatography |
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| Authors: | Werner Müller Hans-Jürgen Schuetz Cecilia Guerrier-Takada Patricia E. Cole Russell Potts |
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| Affiliation: | Fakultät für Biologie der Universität Bielefeld, D-48 Bielefeld, GFR;Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA;Department of Chemistry, Yale University, New Haven, Connecticut, USA |
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| Abstract: | A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases. |
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