Cloning and characterization of a cDNA encoding the cytosolic copper/zinc-superoxide dismutase from sweet potato tuberous root |
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| Authors: | Chi-Tsai Lin Kai-Wun Yeh Ming-Ching Kao Jei-Fu Shaw |
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| Affiliation: | (1) Department of Bioengineering, Tatung Institute of Technology, 40 Chungshan North Road, 3rd sec., Taipei, Taiwan;(2) Department of Botany, National Taiwan University, Taipei, Taiwan;(3) Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan;(4) Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan |
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| Abstract: | A full-length cDNA clone encoding a putative copper/zinc-superoxide dismutase (SOD) of sweet potato, Ipomoea batatas (L.) Lam. cv Tainong 57, was isolated from a cDNA library constructed in  gt10 from tuber root mRNA. Nucleotide sequence analysis of this cDNA clone revealed that it comprises a complete open reading frame coding for 152 amino acid residues. The deduced amino acid sequence showed higher homology (78–86%) with the sequence of the cytosolic SOD than that of the chloroplast SOD from other plant species. The residues required for coordinating copper and zinc are conserved as they are among all reported Cu/Zn-SOD sequences. In addition, it lacks recognizable plastic or mitochondrial targeting sequences. These data suggest that the isolated sweet potato clone encodes a cytosolic Cu/Zn-SOD. |
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| Keywords: | cDNA cytosolic Cu/Zn-SOD superoxide dismutase sweet potato Iopomoea batatas (L.) |
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