| 乙基纤维素微胶囊对过氧化氢酶固定化的研究 |
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| 引用本文： | 王玉洁,刘持标,白光月,褚莹,吴子生,张河哲. 乙基纤维素微胶囊对过氧化氢酶固定化的研究[J]. 中国生物化学与分子生物学报, 1995, 11(2): 201-204 |
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| 作者姓名： | 王玉洁 刘持标 白光月 褚莹 吴子生 张河哲 |
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| 作者单位： | 东北师范大学化学系，中国科学院长春应用化学研究所 |
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| 摘 要： | 以乙基纤维素作膜材，用液中干燥法使过氧化氢酶微胶囊化。研究了微胶囊化操作条件对酶活性的影响。通过测定微胶囊化酶的释放曲线，证明微胶囊膜对过氧化氢酶具有较好的固定性能力。固定化酶用于催化底物过氧化氢分解，测定米氏常数为０．５５ｍｏｌ／Ｌ。
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| 关 键 词： | 微胶囊 过氧化氢酶 固定化酶 |
| 收稿时间： | 1995-04-20 |
Study on Immobilization of Catalase with Ethyl Cellulose Microcapsule |
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| Wang Yu-Jie,Liu Chi-Biao,Bai Guang-Yue,Chu Ying,Wu Zi-Sheng,Chang He-Zhe. Study on Immobilization of Catalase with Ethyl Cellulose Microcapsule[J]. Chinese Journal of Biochemistry and Molecular Biology, 1995, 11(2): 201-204 |
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| Authors: | Wang Yu-Jie Liu Chi-Biao Bai Guang-Yue Chu Ying Wu Zi-Sheng Chang He-Zhe |
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| Affiliation: | (Depedment of Chemistry, Nwtheast Norimal University, Changchun 130024 |
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| Abstract: | Catalase was microencapsulated with ethyl cellulose as film material by drying method in liquid. The effection of microencapsulated conditions on the enzyme activity was investigated with hydrogen peroxide as substrate. The release percentage of the catalase was measured and the resuhs showed that the enzyme can be immobiled in this microcapsule film better than those previously reported. It can catalyzes of the decomposition hydrogen peroxide faster with a MichaelisMenton constant of 0. 55 mol/L. |
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| Keywords: | Microcapsule Catalase Immobilization Ethyl cellulose |
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