An unambiguous assignment method by 2D selective-TOCSY-HSQC and selective-TOCSY-DQFCOSY and structural analysis by selective-TOCSY-NOESY experiments of a biantennary undecasaccharide |
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Authors: | Sato Hajime Kajihara Yasuhiro |
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Institution: | a Bruker Biospin K.K., 3-21-5 Ninomiya, Tsukuba, Ibaraki 305-0051, Japan b Graduate School of Integrated Science, Yokohama City University, 22-2 Seto Kanazawa-ku, Yokohama 236-0027, Japan |
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Abstract: | In this paper we present a newly developed 2D selective-TOCSY-HSQC experiment for unambiguous assignment of individual sugar components of a biantennary undecasaccharide. The correlation signals between the protons and carbons of a selectively excited sugar component in the undecasaccharide were observed in a 2D selective-TOCSY-HSQC experiment. The network of proton signals of this sugar was observed in a 2D selective-TOCSY-DQFCOSY experiment. These two experiments enabled us to perform unambiguous sequential assignment of both protons and carbons of the individual sugar components in the undecasaccharide. In addition, a three-dimensional structural analysis was performed by a 2D selective-TOCSY-NOESY experiment, which clearly distinguishes between inter-sugar and intra-sugar ring NOE signals. |
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Keywords: | Biantennary undecasaccharide NMR spectroscopy Selective-TOCSY-HSQC Selective-TOCSY-DQFCOSY Selective-TOCSY-NOESY |
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