Major O-glycans from the nest of Vespula germanica contain phospho-ethanolamine |
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Authors: | Maes Emmanuel Garénaux Estelle Strecker Gérard Leroy Yves Wieruszeski Jean-Michel Brassart Colette Guérardel Yann |
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Institution: | a Unité de Glycobiologie Structurale et Fonctionnelle, CNRS UMR 8576, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d’Ascq, France b UMR 8525 CNRS, Université de Lille II-Institut de Biologie de Lille et Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP447, F-59021 Lille, France |
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Abstract: | We describe here the structural deciphering of four wasp O-glycans. Following purification of a mixture of glycoproteins from nests of the common wasp Vespula germanica L. (Hymenoptera), their substituting O-glycans were liberated by reducing β-elimination and characterised using a combination of high resolution NMR and mass spectrometry analyses. Besides ubiquitously found in the insect cells GalNAc-ol and Gal(β1-3)GalNAc-ol compounds, two novel O-glycans carrying a 2-aminoethyl phosphate group were described for the first time here. We suggest that they present the following structures: Etn-P-(O→6)-GalNAc-ol and Etn-P-(O→6)-Gal(β1-3)]GalNAc-ol. In conjunction with previous studies, these results suggest that a 2-aminoethyl phosphate group may act as an alternative to sialic acid for conferring charges to glycoproteins. |
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Keywords: | Insect O-Glycan Phosphoethanolamine (PEtn) NMR |
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