A pH-metrical method of determining glucose-6-phosphatase activity |
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Authors: | M B Murataliev E N Vulfson |
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Affiliation: | A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR |
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Abstract: | A simple, rapid, and reproducible method of determining glucose-6-phosphatase activity is described. The glucose 6-phosphate hydrolysis is accompanied by the disappearance of the protons from the medium owing to a phosphate species pK change from 6.1 (in glucose 6-phosphate) to 6.9 (in inorganic phosphate). Alkalization is registered by a pH meter with a recorder. The method described in this paper may be used in routine determinations of glucose-6-phosphatase activity. |
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