Isolation of cDNAs for mouse phenol and bilirubin UDP-glucuronosyltransferases and mapping of the mouse gene for phenol UDP-glucuronosyltransferase (Ugtla1) to chromosome 1 by restriction fragment length variations |
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| Authors: | Osamu Koiwai Kayo Hasada Yoshihiro Yasui Yuka Sakai Hiroshi Sato Tomomasa Watanabe |
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| Institution: | (1) Department of Biochemistry, Aichi Cancer Center Institute, Chikusa-ku, Kanokoden 1-1, 464 Nagoya, Japan;(2) Department of Ultrastructure Research, Institute for Developmental Research, Aichi Prefecture Colony, 480-03 Kasugai, Aichi, Japan;(3) Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, 480-03 Kasugai, Aichi, Japan;(4) Department of Biology, Shiga University of Medical Sciences, 520-21 Ootsu, Shiga, Japan;(5) Present address: Faculty of Veterinary Medicine, Hokkaido University, 060 Sapporo, Japan |
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| Abstract: | The mouse gene for phenol UDP-glucuronosyltranferase (UDPGT;Ugtla1) was mapped at 42 cM on chromosome 1, a position identical to that of the gene for bilirubin UDPGT (Ugtla1), from linkage analysis of a three-point cross test withIdh-1, En-1, andUgtlal as marker genes. The cDNAs for mouse phenol and bilirubin UDPGTs, isolated after amplification by PCR, shared an identical 3 -half region. Our results strongly suggest that mouse bilirubin and phenol UDPGTs are expressed from a single gene and involve alternative splicing events. We also detected duplication of the gene for phenol UDPGT in all mouse strains examined with the exception of MOL-MIT and SUB-SHH. |
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| Keywords: | chromosome mapping mouse phenol UDP-glucuronosyltransferase restriction fragment length variation alternative splicing gene duplication |
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