The Cardiac Copper Chaperone Proteins Sco1 and CCS are Up-Regulated,but Cox 1 and Cox4 are Down-Regulated,by Copper Deficiency |
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Authors: | Jean Getz Dingbo Lin Denis M Medeiros |
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Institution: | (1) Department of Human Nutrition, Kansas State University, 213 Justin Hall, Manhattan, KS 66506, USA |
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Abstract: | Copper is ferried in a cell complexed to chaperone proteins, and in the heart much copper is required for cytochrome c oxidase (Cox). It is not completely understood how copper status affects the levels of these proteins. Here we determined
if dietary copper deficiency could up- or down-regulate select copper chaperone proteins and Cox subunits 1 and 4 in cardiac
tissue of rats. Sixteen weanling male Long–Evans rats were randomized into treatment groups, one group receiving a copper-deficient
diet (<1 mg Cu/kg diet) and one group receiving a diet containing adequate copper (6 mg Cu/kg diet) for 5 weeks. Hearts were
removed, weighed, and non-myofibrillar proteins separated to analyze for levels of CCS, Sco1, Ctr1, Cox17, Cox1, and Cox4
by SDS–PAGE and Western blotting. No changes were observed in the concentrations of CTR1 and Cox17 between copper-adequate
and copper-deficient rats. CCS and Sco1 were up-regulated and Cox1 and Cox4 were both down-regulated as a result of copper
deficiency. These data suggest that select chaperone proteins and may be up-regulated, and Cox1 and 4 down-regulated, by a
dietary copper deficiency, whereas others appear not to be affected by copper status. |
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