Integration of the cyanobacterial <Emphasis Type="Italic">DesA</Emphasis> gene for Δ12-acyl-lipid desaturase improves potato tolerance to paraquat-induced oxidative stress |
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Authors: | I N Demin N V Naraikina V D Tsedendambaev I E Moshkov T I Trunova |
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Institution: | (1) Environmental Biotechnology Research Center, KRIBB, Eoeun-dong 52, Yuseong-gu, Daejeon, 305-806, Korea;(2) Department of Biology, Chungnam National University, Daejeon, 305-764, Korea;(3) Department of Environmental Horticulture, University of Seoul, Seoul, 130-743, Korea; |
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Abstract: | The effect of potato plant (Solanum tuberosum L., cv. Desnitsa) transformation with the desA gene from Synechocystis sp. PCC 6803, encoding Δ12 acyl-lipid desaturase, on the development of plant tolerance to oxidative stress was studied.
To initiate oxidative stress, plants were treated with 1 mM paraquat; this treatment enhanced oxidative processes in both
wild-type and transformed potato plants via the activation of superoxide anion-radical generation. This resulted in the activated
oxidation of membrane lipids and the formation of a great amount of fatty acids with coupled double bonds (conjugated dienes,
CD), further breakdown of lipid molecules, and enhanced production of MDA in tissues of wild-type and transformed plants.
The characteristics of oxidative stress, including lipid peroxidation, were less pronounced in transformants as compared with
wild-type plants. After treatment with paraquat, activities of main antioxidant enzymes (superoxide dismutase, catalase, and
peroxidase) were much higher in wild-type than in transformed plants. Thus, expression of inserted heterologous desA gene for Δ12 acyl-lipid desaturase in potato plants resulted in improved tolerance of transformants to oxidative stress due
to the more efficient maintenance of stable cell membrane structure functioning, and this permits prevention of electron “jump”
to oxygen and, as a result, of accelerated ROS generation. More developed and regularly arranged chloroplast membrane system
in transformants may also favor their improved tolerance. |
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