Continuous Production of Dextran from Immobilized Cells of <Emphasis Type="Italic">Leuconostoc mesenteroides</Emphasis> KIBGE HA1 Using Acrylamide as a Support |
| |
Authors: | Shah Ali Ul Qader Afsheen Aman Abid Azhar |
| |
Institution: | (1) The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan |
| |
Abstract: | The cells of L. mesenteroides KIBGE HA1 were immobilized for the production of dextran on acrylamide gel and gel concentration was optimized for maximum
entrapment. Sucrose at substrate concentration of 10% produced high yield of dextran at 25°C with a percent conversion of
5.82 while at 35°C it was 3.5. However, increasing levels of sucrose diminished dextran yields. The free cells stopped producing
dextran after 144 h, while immobilized cells continued to produce dextran even after 480 h. Molecular mass distribution of
dextran from free cells indicate that it is identical to that of blue dextran while the molecular mass of dextran from immobilized
cells is lower than that of free cells. |
| |
Keywords: | Immobilization Acrylamide Dextran Leuconostoc mesenteroides |
本文献已被 PubMed SpringerLink 等数据库收录! |