Enhanced rapamycin production in Streptomyces hygroscopicus by integrative expression of aveR, a LAL family transcriptional regulator |
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| Authors: | Minhong Huang Min Li Zhixiang Feng Yang Liu Yiwen Chu Yongqiang Tian |
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| Affiliation: | (1) Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, 24 South Section 1, Yihuan Road, Chengdu, Sichuan, 610065, China;(2) College of Life Science and Engineering, Southwest Jiaotong University, 111, North Section 1, Erhuan Road, Chengdu, Sichuan, 610031, China;(3) Sichuan Industrial Institute of Antibiotics, No. 168 Zhonghe Road, Longtan Urban Industry Concentrate Zone, 2nd Section, East 3rd Road, Chengdu, Sichuan, 610052, China; |
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| Abstract: | Rapamycin produced by Streptomyces hygroscopicus displays immunosuppressive, antifungal, anti-tumor, anti-inflammatory and neuro-regenerative properties. To enhance production of rapamycin, aveR, a stimulator in Streptomyces avermitilis, was integrated into the chromosome of S. hygroscopicus TYQ0915. This resulted in a 274.9% increase of rapamycin production in an exconjugant S. hygroscopicus AVH1124. Introduction of aveR acts pleiotropically by affecting growth and sporulation of S. hygroscopicus AVH1124, although aveR is verified to be a pathway-specific regulator in S. avermitilis. This study proved that introduction of a homologous regulatory gene from the same protein family enhanced rapamycin production. |
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