Isolation and characterization of valine-resistant mutants of Nicotiana plumbaginifolia |
| |
Authors: | A Marion-Poll C Missonier J Goujaud M Caboche |
| |
Institution: | (1) Laboratoire de Biologie Cellulaire, INRA, F-78000 Versailles, France |
| |
Abstract: | Summary Haploid mesophyll protoplasts of Nicotiana plumbaginifolia were mutagenized by UV-irradiation. Protoplast-derived colonies were then selected for valine resistance on a medium containing 5 or 10 mM valine. From the resistant calli, plants were regenerated. Resistance was inherited as a recessive Mendelian character in seven clones. Mutations conferring valine resistance were shown to be allelic. Protoplast-derived cells of L-valine-resistant plants were also resistant to L-threonine. Resistance to valine was based on a reduced valine uptake rate. |
| |
Keywords: | Valine resistance Nicotiana plumbaginifolia Protoplast Amino acid uptake |
本文献已被 SpringerLink 等数据库收录! |