Separating lysozyme from bacteriophage P22 in two-phase aqueous micellar systems |
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Authors: | Kamei Daniel T King Jonathan A Wang Daniel I C Blankschtein Daniel |
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Affiliation: | Department of Chemical Engineering, Room 66-444, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. |
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Abstract: | This communication demonstrates that two-phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C(10)E(4)) micellar system increases the yield of a model net positively charged protein, lysozyme, in the micelle-rich phase from 75 to 95%, while still maintaining approximately the same yield of a model net negatively charged virus, bacteriophage P22, in the micelle-poor phase (97% vs. 98%). |
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Keywords: | separation proteins viruses two‐phase aqueous micellar systems viral clearance gene therapy |
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