Streptococcus pneumoniae heat shock protein 70 does not induce human antibody responses during infection |
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Authors: | Kolberg J Høiby E A Aase A Sletten K Rødal G Michaelsen T E Bucher A |
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Institution: | Department of Vaccinology, National Institute of Public Health, Torshov, Oslo, Norway. jan.kolberg@folkehelsa.no |
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Abstract: | Mouse monoclonal antibodies (mAbs) were developed against Streptococcus pneumoniae in search for potential common pneumococcal proteins as vaccine antigens. mAb 230,B-9 (IgG1) reacted by immunoblotting with a 70-kDa protein which was isolated by immunoaffinity chromatography and subsequent preparative electrophoresis. N-terminal amino acid sequencing showed homology to that of heat shock protein 70 (hsp70). The hsp70 epitope reactive with mAb 230,B-9 was found in all the pneumococci examined as well as in other streptococci and enterococci. The epitope was not expressed in several other examined Gram-positive or -negative bacteria. Pneumococcal hsp70 has by other investigators been proposed to be a vaccine candidate. Binding experiments using flow cytometry showed that the epitope was not surface-exposed on live exponential phase grown S. pneumoniae. Human patient sera did not react with affinity-purified pneumococcal hsp70. Therefore the pneumococcal hsp70 does not seem to be of special interest in a vaccine formulation. The human sera contained antibodies to high molecular proteins co-purified with hsp70. Some of these proteins could be the pneumococcal surface protein A. |
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Keywords: | Monoclonal antibody Heat shock protein Vaccine Streptococcus pneumoniae |
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