Monoclonal antibodies to the hydrogenase ofThiocapsa roseopersicina |
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Authors: | Dr Gábor J Tigyi Kornél L Kovács |
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Institution: | (1) Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701 Szeged, Hungary |
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Abstract: | Monoclonal antibodies were produced to electrophoretically pure hydrogenase fromThiocapsa roseopersicina. Protein immunoelectroblotting was used to identify the hydrogenase-specific antibodies. Among the 18 monoclonal antibodies selected by enzyme immunoassay, three were found to react with highly immunogenic trace contaminating proteins. One cell line produced antibody that inhibitied hydrogenase activity. This was the first specific inhibitor of the hydrogenase function. The results suggest that monoclonal antibodies could provide valuable new informations about the enzyme structure as well. |
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