Glycan structures of ocular surface mucins in man,rabbit and dog display species differences |
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Authors: | Louise Royle Elizabeth Matthews Anthony Corfield Monica Berry Pauline M Rudd Raymond A Dwek Stephen D Carrington |
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Institution: | (1) Department of Biochemistry, Glycobiology Institute, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;(2) Ludger Ltd, Culham Science Centre, Abingdon, Oxfordshire, OX14 3EB, UK;(3) Vet Science Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland;(4) Mucin Research Group, Clinical Science at South Bristol, University of Bristol, Bristol, BS2 8HW, UK;(5) Dublin–Oxford Glycobiology Laboratory, NIBRT, Conway Institute, University College Dublin, Belfield, Dublin, Ireland; |
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Abstract: | The composition of the mucus gel of the tear film reflects the competing needs for transparency, stability, hydration, and
protection of the ocular surface. Mucins form the macromolecular scaffolding of this hydrated gel, and glycans decorating
these glycoproteins represent a rich source of binding ligands that may both modulate microbial binding and regulate the physicochemical
characteristics of the gel. This study compares the structure of O-linked glycans derived from the ocular mucins of three
species, to determine whether the ocular surface microenvironment dictates the need for a common pattern of O-linked carbohydrate
structures. Ocular mucus aspirates were collected from healthy humans, rabbits and dogs. Mucins were purified using standard
protocols. O-glycans were released by hydrazinoloysis and subsequently analysed by a combination of HPLC, exoglycosidase digestions
and LC–MS/MS. A total of 12 different O-glycans were identified. In human ocular mucin, the majority were negatively charged
and terminated in sialic acid, whilst those from rabbit or dog were mainly neutral and terminated in α 1-2 fucose and/or α
1-3 N-acetylgalactosamine. The glycans were short: the most common structures being tetra-, tri- or disaccharides. Less elaborate
glycan structures are encountered at the ocular surface than at many other mucosal surfaces. Species-specific glycan expression
is a feature of ocular surface mucins, and has implications for their defensive properties where different microbial and environmental
challenges are encountered.
Louise Royle and Elizabeth Matthews contributed equally to this work. |
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Keywords: | Ocular Mucin Glycans Comparative study |
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