An oxygen-18 tracer investigation of the mechanism of -inositol oxygenase |
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Authors: | Richard Moskala C.Channa Reddy Robert D. Minard Gordon A. Hamilton |
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Affiliation: | Department of Chemistry The Pennsylvania State University University Park, PA 16802 USA |
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Abstract: | In the conversion of -inositol to D-glucuronic acid catalyzed by -inositol oxygenase only one atom of 18O from 18O2 is incorporated into the product, and it is found exclusively in the carboxyl group. Control experiments indicate that under the reaction conditions no exchange of solvent oxygens with D-glucuronate occurs. To avoid exchange during isolation and analysis the oxygenase product was enzymically reduced to L-gulonate and isolated in that form. The results eliminate one possible mechanism for the oxygenase reaction, but are consistent with two others which seem chemically reasonable. |
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Keywords: | To whom correspondence should be addressed |
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