Studies on NADH-cytochrome reductase activities in hemolysates of human and rabbit red cells by isoelectric focusing |
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Authors: | Toshitsugu Yubisui Minoru Tamura Masazumi Takeshita |
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Institution: | Department of Biochemistry, Medical College of Oita, Hazama-cho, Oita 879-56, Japan |
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Abstract: | NADH-cytochrome reductase activities in hemolysates of young and old human erythrocytes, and in hemolysates of rabbit reticulocytes and erythrocytes were measured after the separation of the enzyme from the bulk of hemoglobin only by isoelectric focusing. In any cases, a single main peak of the enzyme activity was detected after the electrophoresis in the fraction with pH 6.8 and 8.3 for human and rabbit red cells, respectively. The rabbit enzyme showed more than 30 times higher enzyme activity than that of human erythrocytes under the standard assay conditions. Significant differences of Micahelis constants for cytochrome of the enzyme were found between young and old human erythrocytes, and also between human and rabbit red cells. |
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