Identification of immunogens of by protein blotting |
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Authors: | Ping C Hu Yuan-shen Huang Judith A Graham Donald E Gardner |
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Institution: | 1. Northrop Services Inc.-Environmental Sciences, Research Triangle Park, N.C. 27709, U.S.A.;2. Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, N.C. 27711, U.S.A. |
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Abstract: | Proteins of were separated by SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose sheet by blotting. Sera obtained from infected hamsters and immunized rabbits were then incubated with the nitrocellulose strips. Proteins which are capable of eliciting antibodies were detected by indirect immunoradioautography using 125I-labeled antisera against hamster or rabbit IgG. Antibodies to seven immunogens were demonstrated in the sera of hamsters infected with by inhalation, while many more proteins were found to be capable of stimulating antibodies in rabbits immunized parenterally with mycoplasmas. This method should prove useful for identifying those components of a microorganism which elicit antibody responses and contribute to the protective state. |
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Keywords: | All correspondence should be addressed to: Ping-chuan Hu Ph D Northrop Services Inc -Environmental Sciences P O Box 12313 Research Triangle Park N C 27709 |
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