Conformational changes of bacteriorhodopsin detected by Fourier transform infrared difference spectroscopy |
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| Authors: | Kenneth J Rothschild Mark Zagaeski William A Cantore |
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| Institution: | 1. Department of Physics Boston University Boston, MA 02215 USA;2. Department of Physiology Boston University Boston, MA 02215 USA |
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| Abstract: | We report the first Fourier transform infrared difference spectra of purple membrane. Evidence is presented that alterations in the vibrations of both the retinylidene chromophore and the protein groups of bacteriorhodopsin associated with photocycling can be detected. This method provides a new tool for probing the conformational changes occurring in bacteriorhodopsin during the proton pump cycle. |
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